The assembly domain of the small capsid protein of Kaposi's sarcoma-associated herpesvirus.
نویسندگان
چکیده
Self-assembly of Kaposi's sarcoma-associated herpesvirus capsids occurs when six proteins are coexpressed in insect cells using recombinant baculoviruses; however, if the small capsid protein (SCP) is omitted from the coinfection, assembly does not occur. Herein we delineate and identify precisely the assembly domain and the residues of SCP required for assembly. Hence, six residues, R14, D18, V25, R46, G66, and R70 in the assembly domain, when changed to alanine, completely abolish or reduce capsid assembly.
منابع مشابه
Small capsid protein pORF65 is essential for assembly of Kaposi's sarcoma-associated herpesvirus capsids.
Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent for KS tumors, multicentric Castleman's disease, and primary effusion lymphomas. Like other herpesvirus capsids, the KSHV capsid is an icosahedral structure composed of six proteins. The capsid shell is made up of the major capsid protein, two triplex proteins, and the small capsid protein. The scaffold protein and the protea...
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ورودعنوان ژورنال:
- Journal of virology
دوره 86 21 شماره
صفحات -
تاریخ انتشار 2012